Calcein AM is a membrane-permeant, non-fluorescent form of calcein that enters cells passively. Once inside the cytosol of cells, Calcein AM is converted to green-fluorescent Calcein by ubiquitous esterases in viable cells, resulting in uniform cytosolic fluorescence (Ex/Em 495 nm/515 nm)

A simple, rapid, and versatile solution for detecting live (green) and dead (red) cells within a population.

Excitation:*	494 ± 3 nm
Emission:*	517 ± 3 nm