Calcein AM is a membrane-permeant, non-fluorescent form of calcein that enters cells passively. Once inside the cytosol of cells, Calcein AM is converted to green-fluorescent Calcein by ubiquitous esterases in viable cells, resulting in uniform cytosolic fluorescence (Ex/Em 495 nm/515 nm)
Ethidium homodimer I (EthD-I) is a membrane-impermeable, high-affinity, nucleic acid stain that selectively labels dead cells with compromised plasma membranes.
ION Vital - Viability Assay Kit is a simple, rapid, and versatile solution for detecting live (green) and dead (red)cells within a population. ION Vital - Viability is compatible with fluorescence microscopy, flow cytometry, andplate reader applications.