Measure a key marker in apoptosis and other critical cellular processes
Membrane potential sensitive dyes, such as JC-1 and JC-10, can be used to monitor the loss of mitochondrial membrane polarization observed during apoptosis. These dyes compartmentalize in healthy mitochondria, leading to the formation of aggregates that exhibit orange fluorescence. When mitochondrial membrane potential is lost during apoptosis, these dyes no longer accumulate and return to green fluorescent monomers. The emission ratio can be measured using plate readers, fluorescence microscopes, or flow cytometers to monitor mitochondrial membrane potential.
MitoVolt is a mitochondrial membrane potential assay that uses JC-10, a potentiometric dye that is more soluble than JC-1. It is an ideal solution for detecting changes in mitochondrial membrane potential due to cell apoptosis or other stress-inducing phenomena, and is compatible with fluorescence microscopy, flow cytometry, and plate reader applications.
