WaveFront Thallium Indicator
100 mM Thallium Solution
Chloride-free Stimulus Solution
High Potassium, Chloride-free Stimulus Solution
1 The use of Probenecid is indicated for some cell types to counteract these cells’ propensity to extrude Brilliant Thallium and other similar indicator dyes resulting in poor dye retention.
2 The TRS reagent is useful for suppressing background resulting from fluorescent compounds in the solutions surrounding the cells. Use of TRS may result in improved signal-to-noise.
3 The concentration of thallium in the stimulus buffer will vary by application. Investigators are encouraged to explore final thallium concentrations ranging from 0.5 to 4.5 mM to achieve the best results. Some targets, typically voltage-gated channels, may benefit from the use of High Potassium Chloride-free Stimulus Solution (Reagent H). Investigators are encouraged to explore mixtures of different proportions of Chloride-free Stimulus Solution and High Potassium, Chloride-free Stimulus Solution to achieve the best results.
4 Data collection typically includes the addition of test compounds or additional reagents to the assay plate during the course of data acquisition. Volumes and concentrations of test compounds and additional reagents vary. Commonly used addition volumes are 5, 10, 20 μL for 384-well plates and 20, 40, and 80 μL/well for 96-well plates.