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ION VITAL - Viability Kit

Viability assay kit is a simple, rapid, and versatile solution for detecting live (green) and dead (red) cells within a population. ION Vital - Viability is compatible with fluorescence microscopy, flow cytometry, and plate reader applications.
ION VITAL: Viability Kit


 
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ION VITAL: Viability Kit
Price: $220.00

PRODUCT CODE: 5000
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ION Vital - Viability Assay Kit





Calcein AM is a membrane-permeant, non-fluorescent form of calcein that enters cells passively. Once inside the cytosol of cells, Calcein AM is converted to green-fluorescent Calcein by ubiquitous esterases in viable cells, resulting in uniform cytosolic fluorescence (Ex/Em 495 nm/515 nm). Calcein, a polyanionic dye, is membrane-impermeant and is well retained within the cytosol of most healthy cells with intact cell membranes.

Ethidium homodimer I (EthD-I) is a membrane-impermeable, high-affinity, nucleic acid stain that is excluded by viable cells with intact cell membranes. When membrane integrity is compromised, a hallmark of dead or dying cells, EthD-I enters the cell and binds DNA, which results in a >30-fold enhancement in bright red, nuclear fluorescence (Ex/Em 528 nm/617 nm). ION Vital—Viability can be used to quantify live and dead cells within eukaryotic cell suspensions or adherent cultures, 3D cultures, organoids, and some non-fixed tissues. Cannot be used for yeast or bacteria.

When following our protocol, ION Vital—Viability kit provides enough reagents to make 150 mL of working solution, enough for fifteen 96-well plates or 1,500 flow cytometry samples. The actual number of assays will vary according to optimal dye concentrations for your application.




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