ION Biosciences Brilliant Calcium Assay is a total assay solution for multi-well plate-based, high-throughput
measurements of dynamic changes in intracellular Ca2+ . The ION Biosciences Brilliant Calcium Assay is useful for
a wide range of effectors of intracellular calcium including many G protein-coupled receptors (GPCRs), divalent
cation channels, and other proteins capable of transporting Ca2+ or affecting intracellular Ca2+ dynamics. In multiwell, plate-based formats, the Brilliant Calcium Assay can be used to discover and characterize the effects of tens to
many tens-of-thousands of compounds and environmental factors which can act to affect intracellular Ca2+ levels.
Over the past 20 years, fluorescence-based measures of intracellular Ca2+ have been widely utilized to discovery
small-molecule modulators of a host of GPRCs, Ion channels and other targets of interest for both drug discovery
and basic research. Inside the ION Brilliant Assay package, you will find all the reagents necessary for preparation of
the Brilliant Calcium Assay for use as a washed or no wash assay for adherent and non- adherent cells. The addition
of a Probenecid solution and an extracellular background masking solution (TRS) offers users the ultimate in
compatibility for cells types which are difficult to load with fluorescent Ca2+ indicators (e.g. Chinese Hamster Ovary,
CHO cells) and when performing assays in complete, serum- containing, cell-culture medium is desired.
About ION’s BRILLIANT CALCIUM MAVERICK , FLEX and EXPRESS Assay Kit: ION Biosciences’ Brilliant Calcium Assay series is a total assay solution for multi-well plate-based, highthroughput measurements of dynamic changes in intracellular Ca2+. Ranging from the Maverick kit - you
supply your own buffers, to the Flex kit – an assay kit Swiss Army Knife, to the Express kit - a premixed
solution to rapidly facilitate your large-scale, high-throughput screening. Each assay kit is designed to be
simple, fast, and reliable fluorescence-based assay.
For years investigators have had only a choice between Molecular Devices’ FLIPR® Calcium 5 (Cal5) and
Calcium 6 (Cal6) Kits and calcium assays such as “Calcium Green” from Thermo Fisher as well (Thermo
F14200 ad Thermo f36206).
Whether your platform is FLIPR® or FlexStation®, FDSS, or Panoptic ION’s assay kits will provide a
smooth process for your FLIPR® assay development and GPCR assays. There are no intermediate washsteps required with these kits and you will find all the reagents necessary for preparation of the Brilliant
Calcium Assay for use as a no wash assay or washed for adherent and non- adherent cells.
Experienced screeners typically have a FLIPR® protocol they are familiar and comfortable with and ION’s
high throughput calcium assay kits are optimized and streamlined to work seamlessly on Hamamatsu
FDSS, FLIPR® Tetra Cellular Screening Systems used today. Compare to Molecular Devices r8190, Thermo
Fisher no wash kits f14217 for example.
Both novice and experienced users will enjoy large signal windows and the lowest lot-to-lot variability
ensuring the highest quality, most consistent results. Evaluation kits are available by request at